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Full text of "A Method of Removing Cuticula from Marine Annelids"

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The following simple method has been found successful for 
the removal of cuticula from marine annelids : 

The worms are first narcotized in sea water to which from 
5 to iojfo commercial alcohol has been added. They are then 
placed in a io^> solution of sodium chloride in distilled water 
for from 24 to 48 hours. The time of immersion in the salt 
solution varies with the different species. The worm is now 
removed to fresh water and an incision with the points of 
scissors is made along the dorsal surface of the animal. A 
little shaking with forceps aided with scalpel is sufficient to 
remove the cuticula entire, although it is usually more conven- 
ient to first cut the worm into pieces. The pieces of cuticula 
thus obtained are floated upon slides and the water is allowed 
to evaporate. In this way permanent mounts are obtained. 
Such mounts are especially valuable for studying the distribu- 
tion of sense organs, gland pores, setae, etc. If the worm has 
been left in the salt solution a sufficient time all the epidermis 
cells will be left behind and a mount of perfectly clean cuticula 
will be obtained. Care must be taken to wash thoroughly in 
fresh water before mounting upon slides; otherwise salt crystals 

will form and injure the preparations. 

Margaret Lewis.